The limits of the detection and quantification can be categorized in three groups:

1. Absolute limits – the lowest number of copies that must present at the beginning of the first cycle to obtain probability of 95 % correct detection;
2. Relative limits – the lowest relative percentage of genetically modified materials that can be detected;
3. Practical limits –the limits applicable to a defined sample.

The specificity of the currently available DNA based methods can be categorized into four groups:

1. Screening methods detecting wide range of GMO without identifying them;
2. Screening methods for a certain type of genetic modification;
3. Construct specific methods sometimes used for identification of GMOs;
4. Transformation specific methods used for identification of GMOs (still under laboratory development; not commercialized).